L-Asparagine/L-Glutamine/ Ammonia Assay Kit
SKU: WZZZ2017
The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials. Includes 100 assays (50 of each).
Compatible with MegaQuant™ Wave Spectrophotometer
Advantages
• Very rapid reaction due to use of uninhibited glutamate dehydrogenase
• All enzymes supplied as stabilised suspensions
• Only kit available
• Very cost effective
• All reagents stable for > 2 years after preparation
• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
• Mega-Calc™ software tool for hassle-free raw data processing
• Standard included
Principle:
(glutaminase)
(1) L-Glutamine + H2O → L-glutamate + NH4+
(microbial glutamate dehydrogenase)
(2) NH4+ + 2-oxoglutarate + NADPH → L-glutamate + NADP+ + H2O
(asparaginase)
(3) L-Asparagine + H2O → L-aspartate + NH4+
Kit size: 50 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 20 min
Detection limit: 0.50 mg/L (L-asparagine) 0.54 mg/L (L-glutamine) 0.06 mg/L (ammonia)