This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials.Includes 72 assays (manual) / 720 assays (microplate).
Compatible with MegaQuant™ Wave Spectrophotometer
Advantages
• Improved assay format (only two absorbance readings required)
• All reagents stable for > 2 years after preparation
• PVP incorporated to prevent tannin inhibition
• Very rapid reaction (~ 4 min)
• Mega-Calc™ software tool for hassle-free raw data processing
• Very competitive price (cost per test)
• Suitable for manual and microplate formats
Principle:
(acetate kinase)
(1) Acetic acid + ATP → acetyl-phosphate + ADP
(phosphotransacetylase)
(2) Acetyl-phosphate + CoA → acetyl-CoA + Pi
(pyruvate kinase)
(3) ADP + PEP → ATP + pyruvate
(D-lactate dehydrogenase)
(4) Pyruvate + NADH + H+ → D-lactic acid + NAD+
Kit size: 72 assays (manual) / 720 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 4 min
Detection limit: 0.254 mg/L
DATA BOOKLET
SDS
MEGA-CALC
Megazyme Knowledge Base
