The Acetic Acid analyzer format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products. On calibration, the prepared reagent is linear to > 28 micrograms of acetic acid per mL of assay solution. Includes 170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL).
The Acetic Acid analyzer format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products. On calibration, the prepared reagent is linear to > 28 micrograms of acetic acid per mL of assay solution. Includes 170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL). The most widely used method for enzymatic quantification of acetic acid is that employing acetyl-coenzyme A synthetase (ACS). However, this method is based on the use of an indicator reaction catalysed by L-malate dehydrogenase that is in permanent equilibrium, and therefore a non-stoichiometric increase in absorbance is observed from the acetate present in the sample. Thus, a slightly non-linear response to increasing acetate concentration is observed upon calibration, resulting in poor R2 values. In addition (depending on the supplier) reagents prepared for auto-analyzer applications can have very limited on-machine stability, due to rapidly increasing blank absorbance values. To overcome these issues, Megazyme developed this alternative acetic acid kit, based on the enzyme acetate kinase (AK; see equations 1–3 below), especially for the auto-analyzer user. This reagent has improved on-machine stability, gives excellent linear calibration curves, and results in a stoichiometric change in absorbance due to the acetic acid present in the sample.
Advantages
• Very stable reagent when prepared for auto-analyzer applications (> 7 days at 4°C)
• PVP incorporated to prevent tannin inhibition
• Linear calibration (R2 ~ 0.9995) up to 30 μg/mL of acetic acid in final reaction solution
• Validated by the University of Wine, Suze la Rousse, France
• Very rapid reaction
• Very competitive price (cost per mL of reagent)
• All reagents stable for > 2 years • Extended cofactors stability
Principle:
(acetate kinase)
(1) Acetic acid + ATP → acetyl-phosphate + ADP
(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate
(D-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → D-lactic acid + NAD+
Kit size: 550 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 10 min
Detection limit: 10 mg/L (recommended assay format)
DATA BOOKLET
SDS
Megazyme Knowledge Base
